Saint,
Just a quick reminder, sharpening is done by increasing the images high frequencies (i.e. the details).
After doing so, as somebody else noticed, you cannot compress your image as well when the details as boosted and the final image (be it png, gif or jpeg) will be bigger.
Also, and this is especially important for medical images, by increasing the high frequencies, you enhance both the real details and the image noise.
If you still want to sharpen your images, check this link. It's a gimp plugin which uses a nifty warping algorithm to squeeze the edges instead of applying brain-dead convolution masks (I am not saying that this is how gimp does it, but I suspect...)
Hope this helps! Anyway, the fact that xsane does not automagically processes the images for you should be seen as a good thing: If you want to apply some image processing to the image (color balance, etc..), then fine! At least, by doing it yourself and being aware you have done so, you won't rely on this shitty stuff manufacturers might have added to their software to hide their product's shortcomings.
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